DiscoMark, our bioinformatics pipeline for marker development and primer design is now published in Mol. Ecol. Res. The program is written in Python, released under a public license (GNU GPL v2), and together with a manual and example data set available at: github.com/hdetering/discomark.
Rutschmann S*, H Detering*, S Simon, J Fredslund, MT Monaghan (in press).
DiscoMark: Nuclear marker discovery from orthologous sequences using draft genome data.
Molecular Ecology Resources 10.1101/1755-0998.12576